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新疆乌鲁木齐HIV—1流行毒株膜蛋白基因C2—V3区序列测定和亚型分析
引用本文:白旭华 管永军. 新疆乌鲁木齐HIV—1流行毒株膜蛋白基因C2—V3区序列测定和亚型分析[J]. 病毒学报, 1997, 13(4): 339-344
作者姓名:白旭华 管永军
作者单位:新疆维吾尔自治区卫生防疫站
摘    要:应用PCR方法对7份1996年3-5月采集于新疆乌鲁木齐HIV-1阳性静脉吸毒者的外周血单核细胞样品进行扩增,获得了HIV-1膜蛋白基因的核酸片段,并对其C2-V3区及邻区350-450个核苷酸序列进行了测定和分析。

关 键 词:亚型分析 序列测定 艾滋病毒-1 毒株 膜蛋白基因

SUBTYPE AND SEQUENCE ANALYSIS OF THE C2-V3 REGION OF GENES OF MEMBRANE PROTEIN AMONG HIV-1 STRAINS IN WULUMUQI EPIDEMIC AREA OF XINJIANG
Bai Xuhua Guan Yongjun Zhang Yuanzhi Shao Yiming Wang Dongli Su Ling Liu Xiaoliang Zhao Quanbi Du Dawei Josef Kostler Hans Wolf. SUBTYPE AND SEQUENCE ANALYSIS OF THE C2-V3 REGION OF GENES OF MEMBRANE PROTEIN AMONG HIV-1 STRAINS IN WULUMUQI EPIDEMIC AREA OF XINJIANG[J]. Chinese journal of virology, 1997, 13(4): 339-344
Authors:Bai Xuhua Guan Yongjun Zhang Yuanzhi Shao Yiming Wang Dongli Su Ling Liu Xiaoliang Zhao Quanbi Du Dawei Josef Kostler Hans Wolf
Affiliation:Bai Xuhua 1 Guan Yongjun 2 Zhang Yuanzhi 1 Shao Yiming 2 Wang Dongli 1 Su Ling 2 Liu Xiaoliang 2 Zhao Quanbi 2 Du Dawei 3 Josef Kostler 4 Hans Wolf 4
Abstract:Seven DNA fragments of HIV-1 env genes were amplified by PCR from uncultured peripheral blood mononuclear cells (PBMCs) obtained from HIV-1 sero-positive drug injecting users (IDUs) in Xinjiang. The C2-V3 region (about 450bp) of them were sequenced. Sequence analysis showed that they are HIV-1 subtypes C, with 1.0% gene divergence among them. The 7 subtype C strains are most close to those found in India and Ruili county of Yunnan, and their nucleotide sequence divergence are both 3.5%. The low level of sequence divergence suggests a recent epidemic of HIV-1 subtype C strain.
Keywords:HIV-1   Subtype C   Sequencing   Xinjiang
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