| 拟青霉属虫生真菌核型的脉冲电泳分析 |
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| 引用本文： | 荣跃文,李春如,樊美珍,李增智.拟青霉属虫生真菌核型的脉冲电泳分析[J].菌物学报,2010,29(3):373-378. |
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| 作者姓名： | 荣跃文 李春如 樊美珍 李增智 |
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| 作者单位： | 1. 安徽农业大学微生物防治省重点实验室,合肥,230036;中国科学院上海生命科学研究院湖州现代农业生物技术产业创新中心,湖州,313000
2. 安徽农业大学微生物防治省重点实验室,合肥,230036 |
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| 基金项目： | 国家高技术研究发展计划（863计划）（No. 2007AA021506）；国家自然科学基金（No. 30570004）；安徽省自然科学基金（No. 070411011） |
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| 摘 要： | 利用钳位均匀电场脉冲电泳系统,从电泳的时间、角度、电压、转换时间间隔等方面优化电泳参数,确定了适宜拟青霉属内4种虫生真菌染色体DNA电泳的最佳条件。根据电泳及软件分析结果,估算出斜链拟青霉和环链拟青霉的染色体数目和染色体组型大小,即核型(karyotype)特征。通过进行拟青霉属内4种7株虫生真菌染色体DNA核型相关的比较和分析,表明拟青霉菌种间染色体数目差异较小,核型差异较大;相同菌种不同菌株间的染色体数、核型大小并不完全相同,存在不同程度的差异;且菌株间核型大小差异程度明显小于菌种间的差异程度。
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| 关 键 词： | 原生质体 钳位均匀电场电泳 染色体组 核型差异 |
The karyotype analysis of the entomopathogenic fungi Paecilomyces spp. by CHEF electrophoresis |
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| Authors: | RONG Yue-Wen LI Chun-Ru FAN Mei-Zhen and LI Zeng-Zhi |
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| Institution: | Anhui Provincial Key Laboratory for Microbial Control, Anhui Agricultural University, Hefei 230036, China
Huzhou Modern Agricultural Biotechnology Innovation Center, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Huzhou 313000, China |
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| Abstract: | The electrophoresis conditions were optimized for chromosome DNA separation of seven strains from four species of the entomopathogenic fungi Paecilomyces spp. by using a CHEF system, including the factors of switching angle, voltage, electrophoresis time and pulse intervals. After gel electrophoresis, the number and molecular weight of chromosomes, i.e. the karyotypes, were analyzed by the software. The results showed that the chromosomal karyotypes were different among the examined species of Paecilomyces spp. and among the strains from the same species. However, the difference level among strains was much lower than that among species. The result of this study provides support for the taxonomy of Paecilomyces spp. |
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| Keywords: | protoplast CHEF electrophoresis genome karyotype diversity |
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