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Colonization of Madagascar periwinkle (Catharanthus roseus), by endophytes encoding gfp marker |
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| Authors: | Adalgisa Ribeiro Torres Welington Luiz Araújo Luciana Cursino Priscilla de Barros Rossetto Mateus Mondin Mariangela Hungria João Lúcio Azevedo |
| Institution: | 1. Departamento de Genética, Escola Superior de Agricultura ‘Luiz de Queiroz’, Universidade de S?o Paulo, PO Box 83, Piracicaba, S?o Paulo, 13400-970, Brazil 2. Embrapa Soja, PO Box 231, Londrina, Paraná, 86001-970, Brazil 3. Departamento de Microbiologia, Instituto de Ciências Biomédicas, Universidade de S?o Paulo, Av. Prof. Lineu Prestes, 1374-Ed. Biomédicas II, Cidade Universitária, S?o Paulo, S?o Paulo, 05508-900, Brazil 4. Department of Biology, Hobart and William Smith Colleges, 300 Pulteney Street, Geneva, NY, USA 5. Instituto de Biologia, Departamento de Genética, Universidade Federal do Rio de Janeiro, Av. Prof. Rodolpho Paulo Rocco s/n Prédio do CCS, 2° Andar, Sala A2-93, Ilha do Fund?o, Rio de Janeiro, RJ, 21949-900, Brazil |
| Abstract: | This study reports the introduction of gfp marker in two endophytic bacterial strains (Pantoea agglomerans C33.1, isolated from cocoa, and Enterobacter cloacae PR2/7, isolated from citrus) to monitor the colonization in Madagascar perinwinkle (Catharanthus roseus). Stability of the plasmid encoding gfp was confirmed in vitro for at least 72 h of bacterial growth and after the colonization of tissues, under non-selective conditions. The colonization was observed using fluorescence microscopy and enumeration of culturable endophytes in inoculated perinwinkle plants that grew for 10 and 20 days. Gfp-expressing strains were re-isolated from the inner tissues of surface-sterilized roots and stems of inoculated plants, and the survival of the P. agglomerans C33:1gfp in plants 20 days after inoculation, even in the absence of selective pressure, suggests that is good colonizer. These results indicated that both gfp-tagged strains, especially P. agglomerans C33.1, may be useful tools to deliver enzymes or other proteins in plant. |
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