长链非编码RNA PVT1在肝癌组织中的表达与临床意义

目的:探讨长链非编码RNA PVT1 (lncRNA-PVT1)在肝癌组织中的表达以及在肝癌诊治中的临床意义。方法:采用qRT-PCR法检测肝癌组织和癌旁肝组织中lncRNA-PVT1的表达情况,通过x2检验分析lncRNA-PVT1的表达水平与肝癌患者临床病理指标之间的相关性,采用Kaplan-Meier法绘制患者术后生存曲线,Log-rank检验比较生存率的差异,单因素和多因素分析评估影响肝癌患者预后的独立危险因素。结果:肝癌组织中lncRNA-PVT1的表达水平显著高于癌旁肝组织(P0.05)。肝癌组织lncRNA-PVT1的表达水平与其Edmondson分级、TNM分期、分化程度和是否发生血管转移具有显著相关性(P0.05),而与患者的年龄、性别、血AFP水平、肿瘤直径、肿瘤数目以及是否有肝炎病史无关(P0.05)。lncRNA-PVT1高表达组患者的术后生存率明显低于lncRNA-PVT1低表达组患者,高表达水平的lncRNA-PVT1、Edmondson分级、TNM分期、分化程度和是否发生血管转移均是影响肝癌患者预后的独立危险因素。结论:lncRNA-PVT1在肝癌组织中呈高表达,高表达水平的lncRNA-PVT1与肝癌患者的临床预后不良密切相关,有望成为今后肝癌治疗的新靶点。

Expression and Clinical Significance of Long Non-Coding RNA PVT1 in the Hepatocellular Carcinoma

ABSTRACT Objective: To investigate the expression of long non-coding RNA PVT1 (lncRNA-PVT1) in the hepatocellular carcinoma and its clinical significance in the diagnosis and treatment of hepatocellular carcinoma(HCC). Methods: The expression level of lncRNA-PVT1 in the hepatocellular carcinoma tissue and adjacent hepatic tissue were detected by qRT-PCR. x² test was used to analyze the correlation between lncRNA-PVT1 expression level and clinicopathological parameters of HCC patients. The postoperative survival curve was drawn by Kaplan-meier method and the comparison of survival rates was performed by Log-rank test. Univariate and multivariate analysis was carried out to assess the independent risk factors affecting the prognosis of HCC patients. Results: The expression level of lncRNA-PVT1 in hepatocellular carcinoma tissues was significantly higher than that of the adjacent hepatic tissue(P<0.05). High level of expression of lncRNA-PVT1 was associated with the Edmondson grading, TNM staging, degree of differentiation and vascular metastasis, and it showed no significant correlation with the age, sex, level of serum AFP, tumor diameter, the number of tumors and the history of hepatitis. The postoperative survival rate of patients with high expression of lncRNA-PVT1 was significantly lower than that in the patients with low expression of lncRNA-PVT1. High expression level of lncRNA-PVT1, Edmondson grading, TNM staging, the degree of differentiation and vascular metastasis are independent risk factors affecting the prognosis of HCC patients. Conclusion: LncRNA-PVT1 is highly expressed in hepatocellular carcinoma, and high expression level of lncRNA-PVT1 is closely related to the poor clinical prognosis of HCC patients, which is expected to be a new target for the treatment of hepatocellular carcinoma in the future.