禾花鲤与建鲤肌间骨miRNAs测序与分析比较

为了研究禾花鲤(Rice flower carp)肌间骨柔软的分子调控机制,采集禾花鲤和建鲤(Jian carp)的肌间骨进行micro RNAs (miRNAs)的高通量测序,并进行了生物信息学分析。从禾花鲤肌间骨的小RNA文库测序获得18 32 nt的高质量miRNA序列25474895条、鉴定出成熟miRNAs 595种,而从建鲤文库获得miRNA序列24625715条、成熟miRNAs 570种。表达差异分析结果显示,与建鲤相比,禾花鲤肌间骨中84个miRNAs呈上调表达、267个呈下调表达。禾花鲤下调表达的miRNAs中有7条为已报道的促进人类成骨的miRNAs,上调表达的miRNAs中有6条为已报道的抑制人类成骨的miRNAs。因此推测禾花鲤可能是通过下调表达促进成骨的miRNAs及上调表达抑制成骨的miRNAs来抑制成骨过程,从而维持其肌间骨细小及柔软的特性。研究为国内首次开展禾花鲤肌间骨发育的分子调控机制研究,为水产动物肌间骨发育的研究奠定了基础。

MIRNAS SEQUENCING AND ANALYSIS OF INTERMUSCULAR BONE BETWEEN RICE FLOWER CARP AND JIAN CARP

In order to study the molecular regulation mechanism of the soft intermuscular bones in Rice flower carp, the intermuscular bones of Rice flower carp and Jian carp were collected for high-throughput sequencing of microRNAs (miRNAs) and bioinformatics analysis. 25474895 and 24625715 high quality sequences of 18—32 nt, as well as 595 and 570 known mature miRNAs were obtained from the small RNA library of Rice flower carp and Jian carp intermuscular bone, respectively. Compared with Jian carp, 84 miRNAs were up-regulated and 267 were down-regulated in the intermuscular bone of Rice flower carp. Seven of the down-regulated miRNAs in Rice flower carp were reported as promoting human osteogenesis. Six of the up-regulated miRNAs were reported as inhibit human osteogenesis. These results indicated that Rice flower carp may inhibit the process of osteogenesis by down-regulating the expression of osteogenic miRNAs and up-regulating the expression of osteogenesis inhibiting miRNAs. Therefore it can maintain its intermuscular bone small and soft characteristics. This study laid the groundwork for the study of molecular regulation mechanism of intermuscular bone development.