Isolation,purification and some properties of a lectin from the winged bean (Psophocarpus tetragonolobus) |
| |
Authors: | T Vijayakumar J A Forrester |
| |
Institution: | (1) Regional Cancer Centre, Medical College Campus, 695 011 Trivandrum, India;(2) Chester Beatty Laboratories, Institute of Cancer Research, Fulham Road, SW3 6JB London, UK |
| |
Abstract: | We have isolated by affinity chromatography a lectin from the seeds of the winged bean (Psophocrapus tetragonolobus) which agglutinated human (group A, B and O), sheep and rabbit, but not mouse erythrocytes. A molecular weight of 41,000 was obtained from gel filtration, and on sodium dodecyl sulphate polyacrylamide gel electrophoresis a single polypeptide chain of molecular weight 35,000 was seen both before and after reduction. Isoelectric focussing of the lectin on polyacrylamide gel gave a single band with a calculated isoelectric point of 4.0. The lectin was found to be rich in acidic amino acids; cysteine was not detected. Carbohydrate analysis revealed no covalently bound sugars.Abbreviations PBS
phosphate-buffered saline
- SDS
sodium dodecyl sulphate
- PAGE
polyacrylamide gel electrophoresis
- WBL
winged bean lectin
- HPLC
high performance liquid chromatography |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|