Additive Effects of Two Different Plasmid-Linked Restriction and Modification Systems in Lactococcus |
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| Authors: | Ping Su Adeline Ng Veronica Kennelly Mary Costello Melissa Harvey Noel Dunn |
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| Institution: | (1) CRC for Food Industry Innovation, Department of Biotechnology, University of NSW, Sydney, Australia, 2052;(2) Gist-Brocades Australia Pty Ltd, PO Box 83, Moorebank, NSW, 2170, Australia |
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| Abstract: | Two plasmids, pND801 and pND802, encoding different restriction and modification systems were isolated from Lactococcus lactis ssp. lactis LL42-1 and Lactococcus lactis ssp. cremoris LC14-1, respectively. pND802 contained one Sphl restriction enzyme site and the whole plasmid was cloned into the Sphl site of the streptococcal/ E. coli shuttle vector pSA3 generating the plasmid pND803. pND803 was stably maintained in L.lactis MG1363 harbouring pND801. The combination of the two R/M systems within L.lactis MG1363 resulted in an additive resistance towards both isometric phage and prolate phage. |
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