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Isolation of the origin of replication of the IncW-group plasmid pSa |
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| Authors: | R C Tait T J Close R L Rodriguez C I Kado |
| Institution: | 1. Davis Crown Gall Group, Departments of Plant Pathology Genetics, University of California, Davis, CA 95616 U.S.A.;2. Departments of Genetics, University of California, Davis, CA 95616 U.S.A. |
| Abstract: | The origin of replication of the IncW plasmid pSa has been cloned and the function of this origin in Escherichia coli examined. A 1.9-kb region of DNA is required for efficient autonomous replication, and a 0.47-kb fragment within this region can initiate replication only in the presence of an autonomously replicating derivative of pSa. An Mr 35,000 protein (repA) is encoded adjacent to the origin and is required for efficient initiation of replication. The derivatives examined provide information suggesting a direct role of partition factors in plasmid replication and incompatibility. |
| Keywords: | Recombinant DNA initiation of replication plasmid incompatibility Gram-negative vectors bp base pairs CAT chloramphenicol acetyltransferase Cm chloramphenicol EtBr ethidium bromide Gm gentamycin kb kilobase pairs Km kanamycin SDS sodium dodecyl sulfate Sm streptomycin Sp spectinomycin Su sulfonamides Tm tobramycin indicates plasmid-carrier state |
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