Preliminary fertility results with frozen buffalo bull semen using tris extender

Various extenders have been proposed to freeze buffalo semen, but much remains to be done in terms of achieving optimum fertility. Fifty semen samples were frozen using tris extender. On an average, 54% and 48% post-freezing motility was observed after 15 minutes and one month storage, respectively. Seventy two buffaloes were inseminated with frozen semen stored at least one month. On the average, a 45.8% first service conception rate was obtained.