Differential gene expression during flowering in the oil palm (Elaeis guineensis) |
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Authors: | R. Singh S.-C. Cheah |
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Affiliation: | (1) Palm Oil Research Institute of Malaysia (PORIM), P.O Box 10620, 50720, Kuala Lumpur, Malaysia e-mail: rajinder@porim.gov.my Fax: 603–8259446, MY |
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Abstract: | The technique of mRNA fingerprinting was used to isolate flower-specific cDNAs in the oil palm. Differences in the RNA populations between vegetative tissue (leaf) and inflorescences at various stages of flower development were examined using 18 primer combinations. A total of 16 flower-specific cDNAs were identified, of which 15 were successfully re-amplified. Reverse Northern analysis confirmed that 8 of the 15 cDNAs appeared to truly represent differentially expressed mRNAs in flowering tissues. Northern blot analysis subsequently showed that 5 of the clones are preferentially or exclusively expressed in the flowering tissues of oil palm. Received: 1 October 1998 / Revision received: 27 September 1999 / Accepted: 11 October 1999 |
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Keywords: | Oil palm Flowering mRNA fingerprinting |
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