Fine genetic mapping of the proximal part of mouse Chromosome 2 excludes Pax-8 as a candidate gene for Danforth's short tail (Sd) |
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Authors: | Haruhiko Koseki Jochen Zachgo Yoko Mizutani Dominique Simon-Chazottes Jean-Louis Guénet Rudi Balling Achim Gossler |
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Institution: | (1) Max-Planck-Institut für Immunobiologie, Stübeweg 51, W-7800 Freiburg, FRG;(2) Max-Delbrück Laboratorium in der Max-Planck-Gesellschaft, Carl von Linné-Weg 10, W-5000 Köln 30, FRG;(3) Unité de Génétique des Mammifères, Institut Pasteur, 25 rue du Docteur Roux, 75724 Paris, Cedex 15, France |
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Abstract: | Danforth's short tail (Sd) is a semidominant mutation of the mouse with effects on the skeleton and the urogenital system. In view of its phenotype and its position in the proximal part of Chromosome (Chr) 2, three genes qualified as possible candidates: Pax-8, a paired box-containing gene; Midkine (Mdk), a retinoic acid-responsive gene; and a new locus (Etl-4) identified by enhancer trapping with a lacZ reporter gene which showed expression in the notochord, the mesonephric mesenchyme, and the apical ectodermal ridge. Three different backcrosses involving all three genes in different combinations were set up and analyzed. From our results we conclude that Sd, Etl-4, Pax-8, and Mdk are independent loci, with Etl-4 being the closest genetic marker (1.1±1.4 cM) to the Danforth's short tail (Sd) gene. |
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