Immunoreactive sites and accumulation of somatomedin-C in rat Sertoli-spermatogenic cell co-cultures |
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| Authors: | L L Tres E P Smith J J Van Wyk A L Kierszenbaum |
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| Affiliation: | 1. New Jersey Medical School, Rutgers Univ., Newark, NJ, USA;2. Department of Medicine, Hematology/Oncology, New Jersey Medical School, Trenton, NJ, USA;3. Graduate School of Biomedical Sciences, Rutgers Univ., Newark, NJ, USA;1. Division of Pediatric Neurology, Department of Pediatrics, Case Western Reserve University, Cleveland, OH 44116, USA;2. Department of Physiology and Biophysics, Case Western Reserve University, Cleveland, OH 44116, USA;3. Department of Psychiatry, Case Western Reserve University, Cleveland, OH 44116, USA;4. Department of Biochemistry, Nencki Institute of Experimental Biology, 02-093 Warsaw, Poland;3. Laboratory of Metabolism, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA;4. National Institute for Infectious Diseases L. Spallanzani, IRCCS, Rome, Italy |
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| Abstract: | ![]()
Sertoli-spermatogenic cell co-cultures prepared from sexually immature rats (20-22 days old) and maintained in serum-free, hormone/growth factor-supplemented medium were used to determine the cell-specific localization of the growth factor somatomedin-C (SM-C). SM-C localization studies were carried out by indirect immunofluorescence using a monoclonal antibody (sm-1.2) to SM-C. In cultured rat hepatocytes, Sertoli and testicular peritubular cells, SM-C immunoreactivity was observed as a diffuse distribution of discrete immunofluorescent granules. Radio-immunoassay experiments using a rabbit antibody against human SM-C showed that testicular peritubular cells and Sertoli cells in primary culture accumulated SM-C in the medium. In spermatogenic cells co-cultured with subjacent Sertoli cells, immunoreactive SM-C was associated with pachytene spermatocytes but not with spermatogonia or early meiotic prophase spermatocytes (leptotene or zygotene). Both Sertoli cells and pachytene spermatocytes displayed binding sites for exogenously added SM-C. SM-C6 binding to spermatocytes reaching an advanced stage of meiotic prophase suggests a possible role of this growth factor in the meiotic process. |
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