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Variation of alkaloid productivity among several clones of hairy roots and regenerated plants ofAtropa belladonna transformed withAgrobacterium rhizogenes 15834 |
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| Authors: | Toshio Aoki Hideki Matsumoto Youichi Asako Yuji Matsunaga Koichiro Shimomura |
| Affiliation: | (1) Tsukuba Medicinal Plant Research Station, National Institute of Health Sciences, 1 Hachimandai, 305 Tsukuba, Ibaraki, Japan;(2) Research Institute for Molecular Genetics, Tsumura and Co., 3586 Yoshiwara, Ami-machi, 300-11 Inashiki, Ibaraki, Japan;(3) Research Planning Division, Marine Biotechnology Inst. Ltd., 2-35-10, Hongo, Bunkyo-ku, 113 Tokyo, Japan;(4) Biochemistry Lab., Kanebo Ltd., 5-3-28 Kotobuki-cho, 250 Odawara, Kanagawa, Japan;(5) Present address: Department of Applied Biological Sciences, Nihon University, 1866 Kameino, 252 Fujisawa, Kanagawa, Japan |
| Abstract: | Hairy root cultures ofAtropabelladonna were established by transformation withAgrobacterium rhizogenes 15834. Five clones of them were employed to study the production of hyoscyamine, the main constituent of the plant, together with other tropane alkaloids. The growth and alkaloid production of each clone were differently affected by basal liquid culture media tested. The transgenic plants regenerated from each clone of the hairy roots had different phenotypes and diverse alkaloid productivity both in the cultured condition and in productivitiy both in the cultured condition and in hydroponics.Abbreviations ANOVA analysis of variance - B5 medium Gamborg B5 medium - BA N6-benzyladenine - B.S. Balanced Solution - dw dry weight - EC electric conductivity - fw fresh weight - GC/MS gas chromatography-mass spectrometry - HPLC high performance liquid chromatography - MS medium Murashige and Skoog medium - NAA naphthalene-l-acetic acid - PCR polymerase chain reaction - SDS sodium dodecyl sulfate - TMS trimethylsilyl - WP medium Woody Plant medium |
| Keywords: | Atropa belladonna Agrobacterium rhizogenes Hairy root cultures Tropane alkaloids |
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