玉米T型细胞质雄性不育系与相应保持系线粒体蛋白质组中的差异蛋白

利用固相pH3—10梯度双向凝胶电泳．对玉米T型细胞质雄性不育系（T—CMS）及其相应保持系叶片（苗期、拔节期、孕穗期），胚轴，胚根和花药（花粉母细胞减数分裂期、花粉粒小孢子单-双核期）线粒体蛋白质组中的差异蛋白进行分析。PDQuest2D图像软件分析表明，苗期和拔节期叶片约有150个蛋白质斑点，胚轴和胚根中可识别出150个线粒体蛋白质斑点，花药中约有100个斑点。利用MALDI—TOF—MS方法．运用MASCOT软件于NCBI进行数据查询．对T—CMS与相应保持系中存在的差异蛋白进行归属鉴定，在T—CMS中存在，保持系中缺失的线粒体蛋白质有：r40cl protein（胚轴中），mature anther—specific protein，DNA—directed RNA polymerase 23kD subunit．hexokinaseⅡ和T—CMS中缺失而在保持系中存在的有：glutathione S—transferase．putative protein。其中T—CMS与相应保持系间．线粒体蛋白质组呈现出差异的组织有胚轴、胚根和小孢子单-双核期的花药。叶片的不同发育时期线粒体蛋白质组呈现明显变化．但T—CMS与保持系间没有差异。在小孢子单-双核期（花粉败育期）的花药中，T—CMS与保持系间线粒体蛋白质组出现明显差异，线粒体蛋白质组出现变异的时期与花粉败育时期相一致。

DIFFERENT PROTEINS IN MITOCHONDRIAL PROTEOME OF T-TYPE MAIZE CYTOPLASMIC MALE-STERILE LINE AND ITS MAINTAINER LINE

Using T-type maize cytoplasmic male sterile line (T-CMS) and maintainer line as experimental materials, we separated mitochondrial proteins from leaves at seedling, shooting, booting stages, mesocotyl, root and anther at meiosis of pollen mother cell, single-double nucleus stage of pollen grain by two-dimensional electrophoresis with immobilized pH3-10 gradients. About 150 mitochondrial protein spots in seedling leaves, 150 spots in mesocotyls, 150 spots in roots and 100 spots in meiosis anther were observed respectively in this investigation. 6 difference protein spots were identified by MALDI-TOF-MS analysis and NCBI database searching. r40c1 protein was present in mesocotyl of T-CMS and absent in maintainer line. Mature anther-specific protein, DNA-directed RNA polymerase 23kDa subunit, hexokinase II were present and glutathione S-transferase, putative polyprotein were absent in pollen aborted anther of T-CMS. Developmental changes in mitochondrial proteins were found in leaves but no differences were observed in T-CMS and its maintainer line. Obvious differences of mitochondrial proteins were found at single-double nucleus stage anther in T-CMS and maintainer line. These different proteins were considered to be associated to pollen aborted in T-CMS.