青蒿毛状根生长、青蒿素合成以及 营养物消耗的动力学

诱导产生的青蒿毛状根培养物置于MS培养基(含30 g/L蔗糖)进行悬浮培养，并对悬浮培养过程中毛状根生长、青蒿素合成、蔗糖、磷酸盐和不同氮源的消耗、pH和电导率的动力学过程进行分析。经30 d培养，生物量干重和青蒿素产量分别达到13.7 g/L和0.23 g/L，碳源和氮源在培养过程中被逐渐利用，而磷酸盐的利用速率最快，培养至15 d所有的磷酸盐均被吸收，pH在培养初期降低，后又逐渐上升，电导率由于毛状根生长对无机离子的吸收而逐渐减低。

Kinetics of artemisinin production,growth and nutrient exhaustion in hairy root cultures of Artemisia annua L.

Hairy root cultures, which were induced from the leaf-disc explants of Artemisia annua L.,were cultured on MS medium with 30 g/L sucrose in shake flasks.During the growth cycle,the time course of fresh and dry weight increase, artemisinin production on a dry weight basis, pH and conductivity as well as the exhaustion of sucrose,nitrogen and inorganic phosphate in the medium was examined. The dry weightand artemisinin production of Artemisia annua L. hairy root cultures reached to 13. 7g/Land 0. 23g/L after 30 days. Total carbohydrate and nitrate was assimilated gradually throughout the growth cycle,but the absorption of phosphate was fast and depleted in 15 days. The pH first dropped and then increased during the growth period. The conductivity of medium decreased in the logarithmic phase due to the exhausiton of inorganic iron by hairy root cultures.