| 肝细胞永生化策略研究进展 |
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| 引用本文： | 赵擎,辛绍杰,貌盼勇.肝细胞永生化策略研究进展[J].生物技术通讯,2007,18(1):107-110. |
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| 作者姓名： | 赵擎 辛绍杰 貌盼勇 |
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| 作者单位： | 1. 解放军总医院,军医进修学院,北京,100853;解放军302医院,传染病研究所病毒室,北京,100039
2. 解放军302医院,传染病研究所病毒室,北京,100039 |
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| 摘 要： | 正常体细胞在细胞分裂过程中由于无法维持端粒的长度和染色体的稳定而衰老、死亡。建立基因稳定、无致瘤性的永生化细胞是永生化研究的目的所在。利用Cre-loxP系统构建的可恢复性永生化细胞可用于以细胞为基础的临床治疗。分化良好并具有肝脏正常合成代谢功能的永生化肝细胞在生物医学研究,尤其是在肝细胞移植、生物人工肝支持治疗以及药理学和毒理学研究方面具有广泛的应用前景。
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| 关 键 词： | 肝细胞 永生化 端粒酶 SV40T抗原 Cre-loxP系统 |
| 文章编号： | 1009-0002（2007）01-0107-04 |
| 修稿时间： | 2006-04-04 |
Advance in Hepatocyte Immortalization Strategies |
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| ZHAO Qing,XIN Shao-jie,MAO Pan-yong.Advance in Hepatocyte Immortalization Strategies[J].Letters in Biotechnology,2007,18(1):107-110. |
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| Authors: | ZHAO Qing XIN Shao-jie MAO Pan-yong |
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| Institution: | 1. General Hospital and Postgraduate Medical College of PLA, Beijing 100853; 2. Department of Virology, 302 Hospital of PLA, Beijing 100039; China |
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| Abstract: | Normal somatic cells have a finite life span due to their inability to maintain telomere length and chromosome stablity. The purpose of immortalization is to create genetically stable, nontumorigenic immortalized cell lines. Using the Cre-loxP recombination and excision system, reversible immortalization has been developing for cell based clinical therapies. Immortalized human hepatocyte cell lines with differentiated liver functions have broad application in biomedical research, especially for hepatocyte transplantation, bioartificial liver support, and pharmacology and toxicology. |
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| Keywords: | hepatocyte immortalization telomerase SV40T antigen Cre-loxP recombination and excision system |
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