Proteomic analysis of CHIKV-infected human fibroblast-like synoviocytes: Identification of host factors potentially associated with CHIKV replication and cellular pathogenesis |
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Authors: | Apamas Sukkaew Ampa Suksatu Sittiruk Roytrakul Duncan R Smith Sukathida Ubol |
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Institution: | 1. Department of Microbiology, Faculty of Science, Mahidol University, Bangkok, Thailand;2. Proteomics Research Laboratory, Genome Institute, 3. National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Pathum Thani, Thailand;4. Center for Emerging and Neglected Infectious Diseases, Mahidol University, Bangkok, Thailand
Institute of Molecular Bioscience, Mahidol University Salaya Campus, Nakorn Pathom, Thailand |
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Abstract: | Chikungunya virus (CHIKV) is a mosquito-borne virus that causes arthralgic fever. Fibroblast-like synoviocytes play a key role in joint damage in inflammatory arthritides and can additionally serve as target cells for CHIKV infection. To gain a better understanding of CHIKV-induced arthralgia, the interaction between CHIKV and synoviocytes was investigated at the protein level. A gel-enhanced liquid chromatography-mass spectrometry (GeLC-MS/MS) approach was used to examine protein expression from primary human fibroblast-like synoviocytes (HFLS) infected with clinical isolates of CHIKV at 12 and 24 hr post infection. Our analysis identified 259 and 241 proteins of known function that were differentially expressed (>1.5 or <−1.5 fold change) following CHIKV infection at 12 and 24 hpi, respectively. These proteins are involved in cellular homeostasis, including cellular trafficking, cytoskeletal organization, immune response, metabolic process, and protein modification. Some of these proteins have previously been reported to participate in arthralgia/arthritis and the death of infected cells. Our results provide information on the CHIKV-induced modulation of cellular proteins of HFLS at an early stage of infection, as well as highlighting biological processes associated with CHIKV infection in the main target cells of the joint. |
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Keywords: | arthralgia/arthritis chikungunya virus GeLC-MS/MS proteomics synoviocyte fibroblast |
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