Development and application of aromatic [13C, 1H] SOFAST-HMQC NMR experiment for nucleic acids |
| |
Authors: | Bharathwaj Sathyamoorthy Janghyun Lee Isaac Kimsey Laura R Ganser Hashim Al-Hashimi |
| |
Institution: | 1. Department of Biochemistry, Duke University, Durham, NC, 27710, USA 2. Department of Chemistry, University of Michigan, Ann Arbor, MI, 48109, USA
|
| |
Abstract: | Higher sensitivity of NMR spectrometers and novel isotopic labeling schemes have ushered the development of rapid data acquisition methodologies, improving the time resolution with which NMR data can be acquired. For nucleic acids, longitudinal relaxation optimization in conjunction with Ernst angle excitation (SOFAST-HMQC) for imino protons, in addition to rendering rapid pulsing, has been demonstrated to yield significant improvements in sensitivity per unit time. Extending such methodology to other spins offers a viable prospect to measure additional chemical shifts, thereby broadening their utilization for various applications. Here, we introduce the 2D 13C, 1H] aromatic SOFAST-HMQC that results in overall sensitivity gain of 1.4- to 1.7-fold relative to the conventional HMQC and can also be extended to yield long-range heteronuclear chemical shifts such as the adenine imino nitrogens N1, N3, N7 and N9. The applications of these experiments range from monitoring real-time biochemical processes, drug/ligand screening, and to collecting data at very low sample concentration and/or in cases where isotopic enrichment cannot be achieved. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|