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DNA-dependent RNA polymerase from Spirochaeta aurantia
Authors:Brenda Allan  Everett P Greenberg  rew Kropinski
Institution:Department of Microbiology and Immunology, Queen's University, Kingston, Ontario, Canada;Department of Microbiology, College of Agriculture and Life Sciences, Cornell University, Ithaca, NY, U.S.A.
Abstract:Abstract A DNA-dependent RNA polymerase was isolated from Spirochaeta aurantia . The M r values of the holoenzyme subunits are 164000, 142000, 84000, and 44500. The RNA polymerase activity was sensitive to heparin, streptolydigin, and actinomycin D, while rifampicin and streptovaricin did not inhibit activity.
Keywords:RNA polymerase              Spirochaeta aurantia            rifampicin resistance
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