| 锌对缺血／再灌注肝脏自由基含量和细胞凋亡的影响 |
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| 引用本文： | Jiang YG,Guo CJ,Wei JY. 锌对缺血／再灌注肝脏自由基含量和细胞凋亡的影响[J]. 中国应用生理学杂志, 2001, 17(3): 244-246 |
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| 作者姓名： | Jiang YG Guo CJ Wei JY |
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| 作者单位： | 军事医学科学院卫生学环境医学研究所, |
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| 摘 要： | 目的：观察补锌对缺血再灌注（HIR）大鼠肝脏自由基含量及细胞凋亡的影响。探讨补锌保护肝损伤的机制。方法：用荧光分光光度法测定血清MDA含量;用电子自旋共振法测定肝脏自由基浓度;用流式细胞术检测肝细胞凋亡。结果：HIR组大鼠血清MDA水平和肝自由基产生均增加,补锌后降低;肝脏缺血再灌注后肝细胞凋亡率达到57.72%,补锌后降低40.85%。结论：减少自由基产生和抑制细胞凋亡是锌保护肝缺血再灌注损伤的重要机制。
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| 关 键 词： | 肝缺血 再灌注损伤 自由基 细胞凋亡 锌 |
| 文章编号： | 1000-6834(2001)03-0244-03 |
| 修稿时间： | 2000-07-19 |
Effects of zinc on the free radical signals and apoptosis in liver with ischemia/ reperfusion injury |
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| Jiang Y G,Guo C J,Wei J Y. Effects of zinc on the free radical signals and apoptosis in liver with ischemia/ reperfusion injury[J]. Chinese journal of applied physiology, 2001, 17(3): 244-246 |
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| Authors: | Jiang Y G Guo C J Wei J Y |
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| Affiliation: | JIANG Yu gang,GU0 Chang jiang,WEI Jing yu,et al . |
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| Abstract: | Aim: In order to explore the preventive mechanism of zinc, the changes of free radical signals and apoptosis in hepatic ischemia/reperfusion injury (HIR) rats were observed. Methods:The MDA levels in serum were measured by fluorophotometry,free radical signals in liver were analyzed with electron spin resonance (ESR) method; and apoptosis was assayed by flow cytometry (FCM). Results: MDA levels in serum and free radica1 contents in liver were both increased in rats with HIR. After zinc supplementation, they were decreased. After HIR, the percentage of subdiploid cells was 57. 72% while it was reduced to 40. 85% after zinc supplementation. Conclusion:Zinc may protect against HIR injury by inhibiting the production of free radicals in liver and hepatocellular apoptosis. |
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| Keywords: | hepatic ischemia/reperfusion injury free radical apoptosis zinc |
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