Toxoplasma gondii: growth in ovine fetal kidney cell cultures |
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Authors: | G N Chang D A Gabrielson |
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Institution: | Department of Bacteriology, North Dakota State University, Fargo, North Dakota 58105, U.S.A. |
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Abstract: | Serial, in vitro passage of Toxoplasma gondii (Rh strain) was successfully performed in a cell line derived from ovine fetal kidney cells. Invasion of this parasite into the kidney cells was easily discernible 1 hr after inoculation. The subsequent proliferation of the parasite was followed in the cytoplasm of the kidney cells. Very active endodyogeny and rosette formations, as many as 13 in a cell, were observed in the cytoplasm of the kidney cells 48 hr postinoculation. After 96 hr of incubation, the parasite population had increased about 132-fold. The virulence of T. gondii against mice was not attenuated after 2 years of in vitro growth which represented 100 serial passages through the kidney cell cultures. Although no "exotoxin" was produced by T. gondii grown in vitro, a Toxoplasma sp. agar gel immunodiffusion test antigen was isolated from the cell-free supernatant fluid of the kidney cell cultures which was identical to an antigen isolated from "toxogenic" organisms harvested from infected mice. |
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Keywords: | Toxoplasmosis Infectivity Mouse Cultivation fetal ovine kidney cell Coccidia Antigen Serology |
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