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Media for cultivation of animal cells: an overview
Authors:Avshalom Mizrahi  Arye Lazar
Affiliation:(1) Department of Biotechnology, Israel Institute for Biological Research, 70450 Ness-Ziona, Israel
Abstract:The increasing interest in products from animal cells has caused an extensive research effort towards development of media for cell cultivation.The basic components in the media used for cultivation of animal cells vary depending upon the characters of the cells and the cultivation method. Basic components consist of an energy source, nitrogen source, vitamins, fats and fatty soluble components, inorganic salts, nucleic acid precursors, antibiotics, oxygen, pH buffering systems, hormones, growth factors and serum. Extensive efforts are directed towards developing serum-free or chemically defined media. Among the serum substitutes is a long list of hormones and growth factors.
Keywords:animal cells  growth factors  mammalian cells  media  serum  tissue culture
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