| Partial characterization of the N－linked oligosaccharide structures on Pselectin glycoprotein ligand－1（PSGL－1） |
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| 引用本文： | Aeed PA,Geng JG,Asa D,Raycroft L,Ma L,Elhammer AP. Partial characterization of the N－linked oligosaccharide structures on Pselectin glycoprotein ligand－1（PSGL－1）[J]. Cell research, 2001, 11(1): 28-36 |
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| 作者姓名： | Aeed PA Geng JG Asa D Raycroft L Ma L Elhammer AP |
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| 作者单位： | [1]PharmaciaCorp.,7000PortageRood,Kalamazoo,MI49001－0199,USA [2]InstituteofBiochemistryandCellBiology,ShanghaiInstituteforBiologicalSciences,ChineseAcademyofSciences,320YueyangRoad,Shangh |
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| 摘 要： |
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| 关 键 词： | 糖蛋白 P－选择蛋白配位子－1 N－聚糖 特征 |
Partial characterization of the N-linked oligosaccharide structures on P-selectin glycoprotein ligand-1 (PSGL-1) |
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| Aeed P A,Geng J G,Asa D,Raycroft L,Ma L,Elhammer A P. Partial characterization of the N-linked oligosaccharide structures on P-selectin glycoprotein ligand-1 (PSGL-1)[J]. Cell research, 2001, 11(1): 28-36 |
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| Authors: | Aeed P A Geng J G Asa D Raycroft L Ma L Elhammer A P |
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| Affiliation: | Pharmacia Corp., Kalamazoo, MI 49001-0199, USA. |
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| Abstract: | PSGL-1, a specific ligand for P-, E- and L-selectin, was isolated from in vivo [3H]-glucosamine labeled HL-60 cells by a combination of wheat germ agglutinin-agarose and P- or E-selectin-agarose chromatography. N-linked oligosaccharides were released from the purified, denatured ligand molecule by peptide: N-glycosidase F treatment and, following separation by Sephacryl S-200 chromatography, partially characterized using lectin, ion-exchange and size-exclusion chromatography in combination with glycosidase digestions. The data obtained suggest that the N-glycans on PSGL-1 are predominantly core-fucosylated, multiantennary complex type structures with extended, poly-N-acetyllactosamine containing outer chains. A portion of the outer chains appears to be substituted with fucose indicating that the N-glycans, in addition to the O-glycans on PSGL-1, may be involved in selectin binding. |
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