Identification of a calmodulin-dependent glycogen synthase kinase in rabbit skeletal muscle, distinct from phosphorylase kinase |
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Authors: | J R Woodgett N K Tonks P Cohen |
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Institution: | First Department of Medicine, University of Tokyo 7-3-1-, Hongo, Bunkyo-ku, Tokyo 113, Japan |
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Abstract: | A glycogen synthase kinase that is completely dependent on Ca2+ and calmodulin has been identified in mammalian skeletal muscle, and purified approximately 3000-fold by chromatography on phosphocellulose and calmodulin--Sepharose. The presence of 50 mM NaCl in the homogenisation buffer was critical for extraction of the enzyme. The calmodulin-dependent glycogen synthase kinase (app. Mr 850 000) is distinct from myosin light-chain kinase and phosphorylase kinase, but phosphorylates the same serine residue on glycogen synthase as phosphorylase kinase. The physiological role of the enzyme is discussed. |
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Keywords: | Tumor promoter Epidermal growth factor Teleocidin Hepatoma cells Retinoic acid Chloroquine |
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