| 番茄子叶外植体转化系统的优化 |
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| 引用本文： | 江晓玲,俞守义,贺竹梅,彭志强,祁瑜. 番茄子叶外植体转化系统的优化[J]. 云南植物研究, 2004, 26(1): 118-120 |
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| 作者姓名： | 江晓玲 俞守义 贺竹梅 彭志强 祁瑜 |
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| 作者单位： | 1. 第一军医大学热带军队卫生学系流行病学教研室,广东,广州,510515
2. 中山大学生命科学院生物工程中心,广东,广州,510275 |
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| 基金项目： | 广东省自然科学基金重点项目(013075) |
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| 摘 要： | 目的 :对番茄子叶外植体的转化进行优化 ,为进行转基因番茄的研究奠定基础。方法 :通过正交设计实验 ,以子叶外植体的存活天数为观察指标 ,分别对农杆菌稀释浓度、浸染时间和转化方式等条件进行优化。结果 :在子叶预培养 2d、活化农杆菌稀释倍数约 30倍、侵染时间为 1 0min、共培养时间为 2d的情况下 ,子叶存活时间最长 ,抗性芽产生频率达 2 5 %。结论 :获得了高效的番茄子叶外植体转化系统 ,为进行基因的转化奠定了基础
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| 关 键 词： | 番茄 子叶外植体 转化系统 |
| 文章编号： | 0253-2700(2004)01-0118-03 |
| 修稿时间： | 2003-06-16 |
To Optimize the Factors that Affected Transformation System of Tomato Cotyledon Explant |
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| JIANG Xiao-Ling,YU Shou-Yi,HE Zhu-Mei,PENG Zhi-Qiang,QI Yu. To Optimize the Factors that Affected Transformation System of Tomato Cotyledon Explant[J]. Acta Botanica Yunnanica, 2004, 26(1): 118-120 |
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| Authors: | JIANG Xiao-Ling YU Shou-Yi HE Zhu-Mei PENG Zhi-Qiang QI Yu |
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| Affiliation: | JIANG Xiao-Ling~1,YU Shou-Yi~1,HE Zhu-Mei~2,PENG Zhi-Qiang~1,QI Yu~2 |
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| Abstract: | Aim:To optimize the factors that affect transformation system of tomato cotyledon explant.Method:By orthogonal design of statistics,factors that affect transformation such as the Agrobacterium concentration,infiltration time and transform style were assembled.Result:Best transformation system could be set up by the contidition of 2 days pre-culture,30-fold-dilution,10-minute-infiltation of Agrobacterium solution,and 2 days co-cultivation.The frenquency of success transformation reached to 25%,higher than average level at present.Conclusion:Factors that affect transformation system of tomato cotyledon explant were successfully optimized. |
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| Keywords: | Tomato Cotyledon explant Transformation system |
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