Jelleines: a family of antimicrobial peptides from the Royal Jelly of honeybees (Apis mellifera) |
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Authors: | Fontana Renato Mendes Maria Anita de Souza Bibiana Monson Konno Katsuhiro César Lílian Mari Marcondes Malaspina Osmar Palma Mario Sergio |
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Institution: | University of Santa Cruz, Ilhéus, BA, Brazil. |
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Abstract: | Four antimicrobial peptides were purified from Royal Jelly of honeybees, by using reverse phase-HPLC and sequenced by using Q-Tof-MS/MS: PFKLSLHL-NH(2) (Jelleine-I), TPFKLSLHL-NH(2) (Jelleine-II), EPFKLSLHL-NH(2) (Jelleine-III), and TPFKLSLH-NH(2) (Jelleine-IV). The peptides were synthesized on-solid phase, purified and submitted to different biological assays: antimicrobial activity, mast cell degranulating activity and hemolysis. The Jelleines-I-III presented exclusively antimicrobial activities against yeast, Gram+ and Gram- bacteria; meanwhile, Jelleine-IV was not active in none of the assays performed. These peptides do not present any similarity with the other antimicrobial peptides from the honeybees; they are produced constitutively by the workers and secreted into Royal Jelly. |
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