Purification and properties of Sandercyanin, a blue protein secreted in the mucus of blue forms of walleye, Sander vitreus |
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Authors: | Chi-Li Yu Daniel Ferraro S Ramaswamy Mark H Schmitz Wayne F Schaefer David T Gibson |
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Institution: | (1) Department of Microbiology, The University of Iowa, 3-403 Bowen Science Building, Iowa City, IA 52242, USA;(2) Department of Biochemistry, The University of Iowa, 4-403 Bowen Science Building, Iowa City, IA 52242, USA;(3) Department of Biological Sciences, University of Wisconsin-Milwaukee, Lapham Hall 181, Milwaukee, WI 53211, USA;(4) Department of Biological Sciences, University of Wisconsin-Washington County, 400 University Drive, West Bend, WI 53095, USA |
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Abstract: | A blue protein present in the mucus coating blue forms of walleye, Sander vitreus, was purified to homogeneity by a combination of ion exchange and hydrophobic interaction chromatography. The purified protein
has a molecular mass of 87,850 and is a homotetramer with a subunit molecular mass of 21,836. Solutions of the protein are
deep blue in color and show absorbance maxima at 383 and 633 nm, respectively. Acetone treatment of the protein releases a
blue chromophore with the spectral characteristics of biliverdin IXα. The results suggest that the blue protein is a new biliprotein
that we have termed Sandercyanin. The function of Sandercyanin is unknown, but limited amino acid sequence analysis suggests
it is a lipocalin that may be involved in the transport of heme degradation products.
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Keywords: | Blue walleye Biliprotein Biliverdin Insecticyanin Lipocalin Tetraodon nigroviridis |
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