Generation of heteroplastidic Nicotiana cybrids by protoplast fusion: analysis for plastid recombinant types |
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Authors: | R Fluhr D Aviv E Galun M Edelman |
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Institution: | (1) Department of Plant Genetics, Weizmann Institute of Science, 76100 Rehovot, Israel |
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Abstract: | Summary Protoplasts of a mutant line of Nicotiana tabacum having a maternally-transmitted chlorophyll deficiency were fused with protoplasts of two alloplasmic-male-sterile Nicotiana lines by the donor-recipient technique. In both fusion experiments variegated plantlets were regenerated which were shown to contain cytoplasms of mixed chloroplast nature. This confirms that with the donor-recipient method one can obtain mixed cytoplasms of genetically different chloroplasts. We present a convenient system to assay for genetic recombination between chloroplasts by combining use of several cytoplasmic markers: vis. chlorophyll pigmentation, chloroplast DNA restriction patterns, tentoxin resistance and male sterility. Within the limits of the experiment no recombinant types were recovered. |
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Keywords: | Tobacco Chloroplast DNA Cybrids Male sterility Tentoxin resistance Plastid recombinants Nicotiana |
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