Characterization of alditol oxidase from Streptomyces coelicolor and its application in the production of rare sugars |
| |
| Institution: | 1. State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu, 214122, China;2. International Joint Laboratory on Food Safety, Jiangnan University, Wuxi, Jiangsu, 214122, China;3. Synergetic Innovation Center of Food Safety and Quality Control of Jiangsu Province, Wuxi 214122, China;1. State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China;2. International Joint Laboratory on Food Safety, Jiangnan University, Wuxi, Jiangsu 214122, China;3. National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, Wuxi, Jiangsu 214122, China;4. School of Biotechnology, Jiangnan University, Wuxi, Jiangsu 214122, China;1. National Engineering Laboratory for Industrial Enzymes, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China;2. University of Chinese Academy of Sciences, Beijing, China;1. State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu, 214122, China;2. International Joint Laboratory on Food Safety, Jiangnan University, Wuxi, Jiangsu, 214122, China;3. Synergetic Innovation Center of Food Safety and Quality Control of Jiangsu Province, Wuxi 214122, China;1. State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu, 214122, China;2. International Joint Laboratory on Food Safety, Jiangnan University, Wuxi 214122, China |
| |
| Abstract: | A synthetic platform for the cascade synthesis of rare sugars using Escherichia coli whole cells was established. In the cascade, the donor substrate dihydroxyacetone phosphate (DHAP) was generated from glycerol by glycerol kinase (GK) and glycerol phosphate oxidase (GPO). The acceptor d-glyceraldehyde was directly produced from glycerol by an alditol oxidase. Then, the aldol reaction between DHAP and d-glyceraldehyde was performed by l-rhamnulose-1-phosphate aldolase (RhaD) to generate the corresponding sugar-1-phosphate. Finally, the phosphate group was removed by fructose-1-phosphatase (YqaB) to obtain the rare sugars d-sorbose and d-psicose. To accomplish this goal, the alditol oxidase from Streptomyces coelicolor (AldOS.coe) was expressed in E. coli and the purified AldOS.coe was characterized. Furthermore, a recombinant E. coli strain overexpressing six enzymes including AldOS.coe was constructed. Under the optimized conditions, it produced 7.9 g/L of d-sorbose and d-psicose with a total conversion rate of 17.7% from glycerol. This study provides a useful and cost-effective method for the synthesis of rare sugars. |
| |
| Keywords: | Alditol oxidase Glycerol Rare sugar Whole cell |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
