Mutagenicity,sister chromatid exchange inducibility andin vitro cell transforming ability of particulates from Athens air |
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Authors: | Kyriacos Athanasiou Iordanis Arzimanoglou Colette Piccoli Hiroshi Yamasaki |
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Institution: | (1) National Hellenic Research Foundation, Biological Research Center, Athens, Greece;(2) Department of Pharmacology Medical School, University of Ioannina, Ioannina, Greece;(3) National Hellenic Research Foundation, International Agency for Research on Cancer, 48, Vas. Constantinou Ave., 116 35, France, Athens |
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Abstract: | Airborne particulates were collected over a period of twelve months by the use of Hi-Vol samplers in the basin of Athens, Greece. N-Hexane extracts were tested in a battery ofin vitro tests for their ability to induce mutation in bacteria as well as mutation, sister chromatid exchange and morphological transformation in cultured mammalian cells. Positive results were found for mutagenicity withSalmonella strain TA98 in the Ames assay, for sister chromatid exchange induction in CHO cells and for transformation in BALB/c 3T3 cells in culture. They also showed weak non-doserelated induction of ouabain resistance in BALB/c 3T3 cells. The contribution of oxidizing and nitrating agents found in the Athens atmosphere, together with sunlight UV irradiation in the formation of direct acting mutagens and potential carcinogens from ambient polycyclic aromatic hydrocarbons, is suggested.Abbreviations FCS
fetal calf serum
- FPG
fluorescent-plus-Giemsa technique
- ouaR
ouabain resistant
- PAH
polycyclic aromatic hydrocarbon
- SCE
sister chromatid exchange
- TSP
total suspended particulate |
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Keywords: | air particulates in vitro transformation mammalian cells mutations Salmonella sister chromatid exchange |
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