Detection of fos protein during osteogenesis by monoclonal antibodies. |
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Authors: | P De Togni H Niman V Raymond P Sawchenko and I M Verma |
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Institution: | Salk Institute, San Diego, California 92138. |
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Abstract: | We have generated monoclonal antibodies by using a synthetic peptide corresponding to amino acid positions 4 to 17 of the human fos protein. The antibodies detected both v- and c-fos proteins by immunoprecipitation, immunoblotting, and indirect immunofluorescence. The monoclonal antibodies not only identified the fos protein complex with the cellular 39-kilodalton protein, but also recognized the modified forms of the mouse, rat, and human fos proteins. In day-17 rat embryos, nuclear-staining fos protein could be identified in the cartilage by immunohistochemical staining. |
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