Endothelial cell adenylate cyclase: activation by catecholamines and prostaglandin I2+ |
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| Authors: | A I Schafer M A Gimbrone R I Handin |
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| Institution: | 1. Department of Medicine Brigham and Women''s Hospital, Boston, MA, USA;2. Department of Pathology Brigham and Women''s Hospital, Boston, MA, USA;3. Harvard Medical School, Boston, MA, USA |
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| Abstract: | Following incubation of intact vascular endothelial cells with 1 mM 3-isobutyl-1-methylxanthine, and isoproterenol or PGI2, cyclic AMP levels increased 4- and 3-fold, respectively. Isoproterenol-stimulated adenylate cyclase activity of cell lysates was selectively inhibited by the β-adrenergic blocking agent propranolol. Catecholamines stimulated adenylate cyclase with the potency series: isoproterenol > epinephrine > norepinephrine. Prostaglandin did not stimulate adenylate cyclase activity in cell lysates, even in the presence of guanine nucleotides or following preincubation of the intact cells with prostaglandins prior to freeze-thaw lysis. |
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