Genetic Variation of the Fc Gamma Receptor 3B Gene and Association with Rheumatoid Arthritis |
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| Authors: | Rute B Marques Mohamed M Thabet Stefan J White Jeanine J Houwing-Duistermaat Aleida M Bakker Gert-Jan Hendriks Alexandra Zhernakova Tom W Huizinga Annette H van der Helm-van Mil Rene E Toes |
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| Institution: | 1. Department of Rheumatology, Leiden University Medical Center, Leiden, The Netherlands.; 2. Department of Internal Medicine, Assiut University Hospital, Assiut, Egypt.; 3. Molecular Development, Murdoch Childrens Research Institute, Melbourne, Australia.; 4. Department of Medical Statistics, Leiden University Medical Center, Leiden, The Netherlands.;Worcester Polytechnic Institute, United States of America |
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| Abstract: | BackgroundFc gamma receptors (FcγRs) play a crucial role in immunity by linking IgG antibody-mediated responses with cellular effector and regulatory functions. Genetic variants in these receptors have been previously identified as risk factors for several chronic inflammatory conditions. The present study aimed to investigate the presence of copy number variations (CNVs) in the FCGR3B gene and its potential association with the autoimmune disease rheumatoid arthritis (RA).Methodology/Principal FindingsCNV of the FCGR3B gene was studied using Multiplex Ligation Dependent Probe Amplification (MLPA) in 518 Dutch RA patients and 304 healthy controls. Surprisingly, three independent MLPA probes targeting the FCGR3B promoter measured different CNV frequencies, with probe#1 and #2 measuring 0 to 5 gene copies and probe#3 showing little evidence of CNV. Quantitative-PCR correlated with the copy number results from MLPA probe#2, which detected low copy number (1 copy) in 6.7% and high copy number (≥3 copies) in 9.4% of the control population. No significant difference was observed between RA patients and the healthy controls, neither in the low copy nor the high copy number groups (p-values = 0.36 and 0.71, respectively). Sequencing of the FCGR3B promoter region revealed an insertion/deletion (indel) that explained the disparate CNV results of MLPA probe#1. Finally, a non-significant trend was found between the novel -256A>TG indel and RA (40.7% in healthy controls versus 35.9% in RA patients; P = 0.08).Conclusions/SignificanceThe current study highlights the complexity and poor characterization of the FCGR3B gene sequence, indicating that the design and interpretation of genotyping assays based on specific probe sequences must be performed with caution. Nonetheless, we confirmed the presence of CNV and identified novel polymorphisms in the FCGR3B gene in the Dutch population. Although no association was found between RA and FCGR3B CNV, the possible protective effect of the -256A>TG indel polymorphism must be addressed in larger studies. |
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