The isolation of abscisic acid (ABA) deficient mutants by selection of induced revertants in non-germinating gibberellin sensitive lines of Arabidopsis thaliana (L.) heynh. |
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| Authors: | M. Koornneef M. L. Jorna D. L. C. Brinkhorst-van der Swan C. M. Karssen |
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| Affiliation: | (1) Department of Genetics, Agricultural University, Wageningen, the Netherlands;(2) Department of Genetics and Plant Physiology, Agricultural University, Wageningen, the Netherlands;(3) Department of Plant Physiology, Agricultural University, Wageningen, the Netherlands |
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| Abstract: | Summary By selecting for germinating seeds in the progeny of mutagen-treated non-germinating gibberellin responsive dwarf mutants of the ga–1 locus in Arabidopsis thaliana, germinating lines (revertants) could be isolated. About half of the revertants were homozygous recessive for a gene (aba), which probably regulates the presence of abscisic acid (ABA). Arguments for the function of this gene were obtained from lines homozygous recessive for this locus only, obtained by selection from the F2 progeny of revertant X wild-type crosses. These lines are characterized by a reduced seed dormancy, symptoms of withering, increased transpiration and a lowered ABA content in developing and ripe seeds and leaves.Abbreviations ABA Abscisic acid - GA4+7 Mixture of gibberellin A4 and A7 - EMS Ethylmethanesulfonate - NG Non-germinating - G Germinating |
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| Keywords: | Arabidopsis thaliana Abscisic acid Gibberellin Physiological mutants Seeds Dormancy Water relations |
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