Immunological detection of propolypeptide of von Willebrand factor on platelet surface |
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| Authors: | K Hashimoto T Usui K Sasaki T Fujisawa F Sekiya J Takagi T Tsukada Y Saito |
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| Institution: | Department of Biological Sciences, Tokyo Institute of Technology, Japan. |
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| Abstract: | Recently we have found that propolypeptide of von Willebrand factor (pp-vWF) obtained from platelets binds to type I collagen. It is known that pp-vWF is present in platelet alpha-granules and is secreted upon activation. In this paper, we demonstrate the two following evidences to show that it is also present on the surface of resting platelets. 1] The antibody against pp-vWF bound to the surface of platelets. 2] The antibody induced aggregation of platelets. The binding of the antibody and the antibody-induced aggregation of platelets were inhibited in a dose-dependent manner by Fab fragment of the antibody. Platelets from von Willebrand disease patients bound less of the antibody and responded weakly to the antibody. |
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