In vitro plant regeneration from organogenic callus of <Emphasis Type="Italic">Curcuma kwangsiensis</Emphasis> Lindl. (Zingiberaceae) |
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| Authors: | Shijun Zhang Nian Liu Aiwu Sheng Guohua Ma Guojiang Wu |
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| Institution: | (1) South China Botanical Garden, Chinese Academy of Sciences, 510650 Guangzhou, China;(2) College of Horticulture and Landscape Architecture, Zhongkai University of Agriculture and Engineering, 510225 Guangzhou, China;(3) Graduate University of the Chinese Academy of Sciences, 100049 Beijing, China; |
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| Abstract: | A novel protocol for callus-mediated shoot regeneration was established for an important medicinal and ornamental plant native
to South China, Curcuma kwangsiensis, using shoot base sections excised from seedlings in vitro as explant sources. The frequency of callus formation reached
91% for explants cultured on MS medium containing 1.4 μM TDZ, 4.4 μM BA and 2.3 μM 2,4-D. 8.2 shoots per callus was achieved
on MS medium supplemented with 1.4 μM TDZ, 17.8 μM BA and 2.7 μM NAA. Single shoots transferred into MS medium free of plant
growth regulator rooted well. Regenerated plants acclimatized ex vitro at 100%, and grew vigorously under shaded greenhouse
conditions. |
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