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Comparative Secretome Analyses of Human and Zoonotic Staphylococcus aureus Isolates CC8, CC22, and CC398
Affiliation:3. Institute for Biology-Microbiology, Freie Universität Berlin, D-14195 Berlin, Germany;4. Center for Biotechnology, Bielefeld University, D-33594 Bielefeld, Germany;5. Robert Koch Institute, Advanced Light and Electron Microscopy, D-13353 Berlin, Germany;6. Institute of Microbiology and Epizootics, Centre for Infection Medicine, Freie Universität Berlin, D-14153 Berlin, Germany;12. Institute of Hygiene, University Hospital Münster, D-48149 Münster, Germany;8. Department of Veterinary Medicine, University of Cambridge, Cambridge CB3 0ES, UK;9. Department Isotope Biogeochemistry, Helmholtz Centre for Environmental Research-UFZ, D-04318 Leipzig, Germany;10. Chair of Geobiotechnology, Technische Universität Berlin, D-13355 Berlin, Germany;123. Institute for Microbiology, University of Greifswald, D-17489 Greifswald, Germany
Abstract:
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  • Highlights
    • •Proteogenomics and secretome comparison of human and zoonotic Staphylococcus aureus lineages.
    • •869 secreted proteins identified in eight S. aureus isolates of CC8, CC22 and CC398.
    • •CC398 lower secretion of surface proteins and higher secretion of hemolysins and exoenzymes.
    • •Regulatory differences in the secretomes could be linked to lower SigB activity in CC398.
    Keywords:Microbiology  Pathogens  Proteogenomics  Secretome  Virulence  clonal complexes  MRSA  Staphylococcus aureus  virulence factor secretion
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