Inorganic polyphosphate hydrolysis catalyzed by skeletal muscular actomyosin complexes is uncoupled with motility |
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| Institution: | 1. Dipartimento di Scienze della Vita e dell''Ambiente, Università Politecnica delle Marche, Ancona, Italy;2. Department of Biochemistry and Biophysics, Stockholm University, Stockholm, Sweden;3. Department of Food, Environmental and Nutritional Sciences, Università degli Studi di Milano, Milan, Italy;1. Interdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh, U.P., India;2. Molecular Science Lab, National Institute of Immunology, New Delhi;3. Chemistry Department, Aligarh Muslim University, Aligarh, U.P., India;4. Moradabad Institutes of Technology, Moradabad, U.P., India;5. Protein Research Group, Institute for Biological Instrumentation of the Russian Academy of Sciences, Institutskaya Str., 7, Pushchino, Moscow Region 142290, Russia;6. Department of Biological Sciences, Faculty of Sciences, King Abdulaziz University, P.O. Box 80203, Jeddah, Saudi Arabia;7. Department of Molecular Medicine, USF Health Byrd Alzheimer''s Research Institute, Morsani College of Medicine, University of South Florida, Tampa, FL, USA |
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| Abstract: | Hydrolysis of the triphosphate moiety of ATP, catalyzed by myosin, induces alterations in the affinity of the myosin heads for actin filaments via conformational changes, thereby causing motility of the actomyosin complexes. To elucidate the contribution of the triphosphate group attached to adenosine, we examined the enzymatic activity of heavy meromyosin (HMM) with actin filaments for inorganic tripolyphosphate (3PP) using a Malachite green method and evaluated using fluorescence microscopy the effects of 3PP on actin filament motility on HMM-coated glass slides. In the presence of MgCl2, HMM hydrolyzed 3PP at a maximum rate of 0.016 s−1 HMM−1, which was four times lower than the hydrolysis rate of ATP. Tetrapolyphosphate (4PP) was hydrolyzed at a rate similar to that of 3PP hydrolysis. The hydrolysis rates of 3PP and 4PP were enhanced by roughly 10-fold in the presence of actin filaments. In motility assays, the presence of polyphosphates did not lead to the sliding movement of actin filaments. Moreover, in the presence of ATP at low concentrations, the sliding velocity of actin filaments decreased as the concentration of added polyphosphate increased, indicating a competitive binding of polyphosphate to myosin heads with ATP. These results suggested that the energy produced by standalone triphosphate hydrolysis did not induce the unidirectional motion of actomyosin and that the link between triphosphate and adenosine was crucial for motility. |
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