Expression from symbiotic promoters ofRhizobium meliloti inAzotobacter vinelandii andAzospirillum brasilense |
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Authors: | Sarasija Hoare H K Das |
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Institution: | (1) Genetic Engineering Unit, School of Environmental Sciences, Jawaharlal Nehru University, 110 067 New Delhi, India |
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Abstract: | InRhizobium meliloti, the promoter P1 of thenif HDK operon, and also the promoter P2, have earlier been shown to be active in the bacteria present in alfalfa root nodules, but
not in the bacteria grown aerobically in culture. Here we have looked at the expression from P1 and P2 in two non-symbiotic
nitrogen-fixing bacteria,Azotobacter vinelandii andAzospirillum brasilense, using constructions in which the promoters are fused upstream of theβ-galactosidase gene. The promoter P1, but not P2, is active inA. vinelandii, while neither P1 nor P2 is active inAzospirillum brasilense. |
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Keywords: | Rhizobium meliloti Azotobacter vinelandii Azospirillum brasilense P1 and P2 promoters |
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