Placental alkaline phosphatase types and subtypes determined by agarose gel electrophoresis and separator isoelectric focusing |
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| Authors: | M Teder J Parik |
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| Institution: | (1) Laboratory of Human Genetics, Department of Genetics and Biotechnology, Institute of General and Molecular Pathology, Tartu State University, 202400 Tartu, Estonia, USSR |
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| Abstract: | Summary Two techniques for phenotyping the human placental alkaline phosphatase system were developed: high-voltage agarose-gel electrophoresis
and thin-layer separator isoelectric focusing on agarose. These methods enabled a more rapid and sensitive phenotyping of
all common phenotypes than the traditionally employed starch-gel electrophoresis. An extended polymorphism of placental alkaline
phosphatase was revealed by isoelectric focusing. The existence of two suballeles of Pl1 allele and two suballeles of Pl2 allele was postulated. |
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