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Rapid isolation of host-independent Bdellovibrio bacteriovorus |
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| Authors: | Ferguson Megan A Schmitt Jaclyn L Sindhurakar Anil R Volle Catherine B Nuñez Megan E Spain Eileen M |
| Institution: | aDepartment of Chemistry, SUNY New Paltz, 1 Hawk Drive, New Paltz, NY, 12561, USA bDepartment of Chemistry, Occidental College, 1600 Campus Rd, Los Angeles, CA, 90041, USA cDepartment of Chemistry, Mount Holyoke College, 50 College Street, South Hadley, MA, 01075, USA |
| Abstract: | A new method of isolating host-independent Bdellovibrio bacteriovorus has been developed. Filtered suspensions of host-dependent cells are dropped in small volumes onto 0.2 μm membranes laid on rich media agar. Significant growth is observed within 1–2 days; these cells were confirmed to be B. bacteriovorus using microscopic observations and PCR. |
| Keywords: | Bdellovibrio bacteriovorus Host-independent Yellow Isolation |
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