| 微波诱变结合化学诱变选育纤维素酶高产菌的研究 |
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| 引用本文： | 兰时乐,李立恒,王晶,赵谦,周馥卉.微波诱变结合化学诱变选育纤维素酶高产菌的研究[J].微生物学杂志,2007,27(1):22-25. |
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| 作者姓名： | 兰时乐 李立恒 王晶 赵谦 周馥卉 |
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| 作者单位： | 湖南农业大学生物科学技术学院,湖南,长沙,410128 |
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| 基金项目： | 湖南农业大学校科研和教改项目 |
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| 摘 要： | 以纤维素酶产生菌TP1202为出发菌株,通过微波诱变和硫酸二乙酯、氯化锂诱变剂进行诱变,选育到1株纤维素酶高产菌株AS5。在适宜条件下,其产生的羧甲基纤维素酶活力(CMC),滤纸酶活力(FPA)和棉花糖酶活力分别是出发菌株的107%、152.4%和140.5%。
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| 关 键 词： | 微波诱变 化学诱变 纤维素酶 绿色木霉 |
| 文章编号： | 1005-7021(2007)01-0022-04 |
| 修稿时间： | 2006年7月20日 |
Breeding of High-Cellulase-Yielding Strain mutated by Microwave and Chemicals |
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| LAN Shi-le,LI Li-heng,WANG Jing,ZHAO Qian,ZHOU Fu-hui.Breeding of High-Cellulase-Yielding Strain mutated by Microwave and Chemicals[J].Journal of Microbiology,2007,27(1):22-25. |
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| Authors: | LAN Shi-le LI Li-heng WANG Jing ZHAO Qian ZHOU Fu-hui |
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| Abstract: | A cellulase-producing strain TP1202 was used as starting strain and mutated by microwave,DES,and LiCl.And a high-cellulase producing strain AS5 was bred.Under suitable conditions,its CMCase activity,FPA activity,and cotton carbohydrase activity were 107%,152.4%,and 140.5% of those of the starting strain respectively. |
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| Keywords: | microwave mutation chemical induction cellulase Trichoderma viride |
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