马立克病病毒群共同性抗原P79蛋白质基因的鉴定和定位

IDENTIFICATION AND LOCALIZATION OF THE MAREK'S DISEASE VIRUS GROUP-COMMON ANTIGEN P79 GENE

Marek's disease virus ( MDV ) 79kD protein ( P79 ) is an antigen com-mon for 3 serotypes of the virus group. By using monoclonal antibody T81 against MDV, a P79-expression recombinant phage clone GB-2 was isolated from the MDV-infected duck embryo fibroblast ( DEF ) cell ge-nomic DNA library which was built in the expression vector E.coli lam-bda gtll phage. Restriction enzyme analysis and polymerase chain reac-tion indicated that the GB-2 clone contained a MDV-origin insert of 2.5kb. As theprobe,the labeled GB-2 clone was hybridized withGA strain MDV-infected DEF genomic DNA digested by different restriction en-zymes and the knowh BamHI-B fragment of MDV genomic DNA in Southern blot. The results demonstrated that the gene encoding the MDV group-common antigen P79 is located in the whole BamHI-R fragment and parts of O and B fragments which were connected with the R fragment on the MDV genomic DNA map.