Preparation and stability of the helical form of poly 2'-O-ethyluridylic acid |
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Authors: | JT Ku?mierek Maria Kielanowska D Shugar |
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Institution: | 1. Institute of Biochemistry & Biophysics, Academy of Sciences, 02532 Warszawa, Poland;2. Dept. of Biophysics, Institute of Experimental Physics, University of Warsaw, 02089 WarszawaPoland |
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Abstract: | 2′ (3′)-O-ethyl-CMP was prepared by alkylation of CMP with diethylsulphate in alkaline medium and deaminated to give 2′(3′)-O-ethyl-UMP, which was phosphorylated to 2′(3′)-O-ethyl-UDP. About 90% of the product consisted of the 2′ isomer. The 2′(3′)-O-ethyl-UDP was readily polymerized by . polynucleotide phosphorylase in the presence of Mn++, but not Mg++. The 3′-isomer did not seriously interfere with polymerization nor did it act as a chain terminator. The resulting poly 2′-O-ethyluridylic acid formed a helical structure with a stability much higher then that of poly (rU) or poly 2′-O-methyluridylic acid. It also complexed readily with poly (rA). Implications with regard to the role of the 2′-hydroxyl in nucleic acid conformation are discussed. |
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