防己抑藻效应及其化感物质的HPLC分析

通过常温水浸提方法获得12种药用植物浸提液，并分别研究其抑藻效应。结果显示：防己水浸提物(相对浓度1 g·L^-1)抑制蛋白核小球藻的效应最强，最大比生长率为-0.28 d^-1。防己甲醇浸提物对蛋白核小球藻的抑藻效应显著，且持续时间长，最低有效抑藻浓度为30 mg·L^-1。HPLC鉴定结果显示：防己水浸提液和甲醇浸提液中主要含有防己诺林碱、粉防己碱等生物碱类化学成分，防己对治理水华发生有较大的应用前景。

Inhibitory Effect of Algae Growth and HPLC Analysis of Allelochemicals from Stephania tetrandra

Aqueous extracts of 12 kinds of medicinal plants were obtained by soak extraction, and their inhibitory effects on algae growth were studied. The results showed that: when the relative concentration was 1 g·L^-1, aqueous extracts of Stephania tetrandra had the strongest inhibitory effect on the growth of Chlorella pyrenoidosa, and the maximum specific growth rate was -0.28 d^-1. The methanol extract of S.tetrandra had significant and long-lasting inhibitory effect on the growth of C.pyrenoidosa and the lowest effective concentration in inhibiting algae was 30 mg·L^-1.The identification results of HPLC showed that: the main constituents in the extracts were alkaloids such as fangchinoline and tetrandrine, etc.S.tetrandra had great prospect of application in controlling water bloom.