旋毛虫感染小鼠肠道菌群变化的研究

目的通过观察黑龙江株旋毛虫感染小鼠肠道分泌物中分泌型免疫球蛋白A、肠道菌群的变化，探讨感染小鼠肠道菌群的变化。方法分别于小鼠感染黑龙江株旋毛虫后7、14、21、28和35d，观察模型组及对照组小鼠肠道分泌物中的分泌型免疫球蛋白A、肠道双歧杆菌、乳酸杆菌、肠杆菌、肠球菌的菌群变化。sIgA采用放射免疫法检测。结果模型组sIgA分泌水平在感染后14d达高峰，随后缓慢下降但始终保持高水平（P〈0．01）。模型组肠道双歧杆菌的数量在感染后7d略低于对照组（P〈0．05），第14天降至最低水平，随后逐渐升高，至感染后35d恢复正常水平。乳酸杆菌的数量在感染后7d略低于对照组，第14天降至最低水平，随后逐渐增加（P〈0．05）。肠杆菌的数量在感染后7d略高于对照组，感染后14d明显高于对照组，随后始终保持下降趋势（P〈0．05）。肠球菌在感染后7d略高于对照组（P〈0．05），在14d明显高于对照组，随后缓慢下降，至感染后35d恢复正常水平。结论旋毛虫感染小鼠sIga的分泌在肠道免疫中发挥重要作用，同时也影响肠道菌群；肠道菌群的变化可能与旋毛虫感染小鼠免疫系统中sIgA的分泌有关。

Changes of intestinal flora in mice with trichinella spiralis infection

Objective To observe the dynamic change of sIgA and intestinal flora of mice infected by Heilongjiang trichinellosis,and study the effect of immune response on intestinal flora.Methods The level of sIgA and changes of Bifidobacteriam,Lactobacillus,Enterobactes and Enterococus in the experimental group and control group were detected respectively at the days 7,14,21,28 and 35.The level of sIgA was detected by the radioimmunity(RI) method.Results The level of sIgA of the experimental group reached the peak at Day 14 and then decresed gradually,but still higher than that of the control group(P<0.01).The level of Bifidobacteriam of the experimental group was lower than that of the control group at Day 7(P<0.05),lowest at Day 14,and then increased gradually to normal.level at Day 35.The level of Lactobacillus of the experimental group was slightly lower than the that of control group at Day 7,lowest at Day 14,and then increased gradually(P<0.05).The level of Enterobactes of the experimental group was slightly higher than that of the control group at Day 7,highest at Day 14,then fell gradually(P<0.05).The level of Enterococus of the experimental group was slightly higher than that of the control group at day 7(P<0.05),highest at Day 14,and then fell gradually to normal level at day 35.Conclusion The sIgA is the important antibody against intestinal infection by Trichinella spiralis and affects intestinal flora of mice.The changes of intestinal flora may be related to the excretion of sIgA.