暗黑鳃金龟甲幼虫肠道细菌分离及鉴定

目的从微生态学角度研究暗黑鳃金龟甲幼虫营养生理活动，探讨其肠道菌群的构成，为其资源开发及生物防治提供理论依据。方法按传统分离方法，从暗黑鳃金龟甲幼虫肠道环境中分离纯化获得10个细菌菌株，对其菌体形态、染色反应、培养性状、生理生化反应进行了系统研究。结果研究结果表明，上述10个细菌菌株分别属于鲁氏耶尔森菌（Yersinia．ruckeri）、侧胞芽胞杆菌（Bacilluslaterosporus）、坚强芽胞杆菌（Bacillus．firmus）、放线杆菌属（Actinobacillus）、飞虫杀雄菌（Arsenophonusllasoniae）、不动杆菌属（Acinetobacter）、气单胞菌Aeromonas）、沙门菌属（Sal—moneUa）、短芽胞杆菌（Bacillusbrevis）、变形菌属（Proteus）。结论通过对暗黑鳃金龟甲幼虫肠道细菌的鉴定，其肠道细菌在培养性状、生理性状、生理生化测定等方面存在较多差异。

The isolation and identification of intestinal bacteria from larval Holotrichia parallela Motschalsky(Coleoptera:Scarabaeidae)

Objective To discuss the composition of intestinal flora of Holotrichia parallela Motschalsky and provide theoretical foundation for its exploitation and biological control.Method Ten bacteria strains were isolated from the intestine of the larval Holotrichia parallela Motschalsky by traditional isolation and purification method.Their morphology,staining reactions,cultural characteristics,physiological and biochemical reactions were studied systematically.Result The 10 bacteria strains belong to Yersinia.ruckeri,Bacillus laterosporus,Bacillus firmus,Actinobacillus,Arsenophonus nasoniae,Acinetobacter,Aeromonas,Salmonella,Bacillus brevis and Proteus.Conclusion The intestinal bacterial flora of the larval Holotrichia parallela Motschalsky have many different characteristics to a certain extent.