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| 脲原体拓扑异构酶基因突变与喹诺酮类耐药的相关性研究 | |
| 引用本文： | 黄亚,冯春燕,李擎天,郭晓奎,董珂.脲原体拓扑异构酶基因突变与喹诺酮类耐药的相关性研究[J].中国微生态学杂志,2012,24(8):699-702. |
| 作者姓名： | 黄亚 冯春燕 李擎天 郭晓奎 董珂 |
| 作者单位： | 1. 解放军第455医院生殖医学中心,上海 200052;上海交通大学医学院病原生物学教研室,上海200025 2. 浙江医学高等医学专科学校基础医学院,浙江杭州,310053 3. 上海交通大学医学院附属瑞金医院检验系,上海,200025 4. 上海交通大学医学院病原生物学教研室,上海200025;上海交通大学医学院附属瑞金医院检验系,上海200025 5. 上海交通大学医学院病原生物学教研室,上海,200025 |
| 基金项目： | “艾滋病和病毒性肝炎等重大传染病防治”科技重大专项(2008ZX10004-002;2009ZX10004-712);浙江省教育厅科研计划项目(Y201122483) |
| 摘 要： | 脲原体对喹诺酮类抗生素的耐药性与拓扑异构酶的基因突变有关。本研究在前期提出的棋盘稀释法脲原体药敏试验的基础上，对拓扑异构酶基因进行序列分析。结合既往的文献资料，确认了ParCS83L是脲原体喹诺酮类耐药性相关突变；而GyrAE112D和ParCT125A属于菌株的多态性表现。同时，本研究还发现了一些新的可能和喹诺酮类耐药相关的基因突变，这些突变以及多个基因突变是否存在叠加效应尚需进一步研究。 |
| 关 键 词： | 脲原体 拓扑异构酶 喹诺酮 gyrA parC |
The relationship between topoisomerase gene mutations and the quinolone resistance in Ureaplasma |
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| HUANG Ya , FENG Chun-yan , LI Qing-tian , GUO Xiao-kui , DONG Ke.The relationship between topoisomerase gene mutations and the quinolone resistance in Ureaplasma[J].Chinese Journal of Microecology,2012,24(8):699-702. | |
| Authors: | HUANG Ya FENG Chun-yan LI Qing-tian GUO Xiao-kui DONG Ke |
| Institution: | 1.Center for Reproductive Medicine,Chinese PLA No.455 Hospital,Shanghai 200052,China;2.Department of Medical Microbiology and Parasitology,Shanghai Jiao Tong University School of Medicine,Shanghai 200025,China;3.Department of Basic Medicine,Zhejiang Medical College,Hangzhou 310053,China;4.Department of Laboratory Medicine,Ruijin Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai 200025,China) |
| Abstract: | The quinolone resistance is related to the topoisomerase gene mutations in ureaplasma.Based on the previous ureaplasma susceptibility test in checkerboard dilution method,the topoisomerase gene sequences were analyzed in this study.Taking the previous studies into consideration,ParC S83L was confirmed as the quinolone resistance gene mutation in ureaplasma;GyrA E112D and ParC T125A were confirmed as gene sequence polymorphisms in ureaplasma.At the same time,some more possible quinolone resistance related topoisomerase gene mutations were found in this study;further studies were needed to focus on these mutations and whether the additive effect of multiple gene mutations in quinolone resistance is present. |
| Keywords: | Ureaplasma Topoisomerase Quinolone gyrA parC |
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