| 肝胚瘤细胞株HepG_2中HBx蛋白对SOCS3表达的影响及其机制 |
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| 引用本文： | 管世鹤,潘颖,杨凯,吴圆圆,杨东亮.肝胚瘤细胞株HepG_2中HBx蛋白对SOCS3表达的影响及其机制[J].中国微生态学杂志,2012,24(5):385-388. |
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| 作者姓名： | 管世鹤 潘颖 杨凯 吴圆圆 杨东亮 |
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| 作者单位： | 1. 安徽医科大学第二附属医院检验科,安徽合肥,230601
2. 华中科技大学同济医学院附属协和医院感染病科,湖北武汉,430022 |
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| 基金项目： | 国家自然科学基金资助(81171662);安徽省卫生厅科学基金资助(2010C057) |
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| 摘 要： | 目的探讨乙型肝炎病毒X蛋白(Hepatitis B virus X protein,HBx)对肝胚瘤细胞株HepG2表达信号抑制因子3(SOCS3)的影响及其机制。方法将表达HBx蛋白的重组质粒FL1-145HBx转染HepG2细胞,以不同浓度的SRC抑制剂PP2处理转染细胞,运用Western blot和RT-PCR技术分析HBx、SOCS3 mRNA和蛋白的表达情况,并以免疫细胞化学分析转染细胞中p-SRC的表达水平。结果重组质粒FL1-145HBx转染HepG2细胞后,转染细胞HBx能够表达HBx蛋白,并且细胞中SOCS3 mRNA和蛋白表达水平随着HBx蛋白的表达而显著增加(P0.05),同时p-SRC蛋白的表达增强;而SRC抑制剂PP2处理转染细胞后,随着p-SRC蛋白表达的减少SOCS3蛋白表达逐渐下降。结论在肝胚瘤细胞株HepG2中,HBx蛋白很可能通过促进SRC蛋白的磷酸化而诱导SOCS3蛋白的表达。
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| 关 键 词： | 乙型肝炎病毒 HBx蛋白 SOCS3蛋白 SRC蛋白 |
The influence of Hepatitis B Virus X Protein on the expression of SOCS3 protein and its mechanisms in HepG2 cells |
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| GUAN Shi-he
,
PAN Ying
,
YANG Kai
,
WU Yuan-yuan
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YANG Dong-liang.The influence of Hepatitis B Virus X Protein on the expression of SOCS3 protein and its mechanisms in HepG2 cells[J].Chinese Journal of Microecology,2012,24(5):385-388. |
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| Authors: | GUAN Shi-he PAN Ying YANG Kai WU Yuan-yuan YANG Dong-liang |
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| Institution: | 1.Department of Laboratory,the Second Affiliated Hospital of Anhui Medical University,Hefei 230601,China;2.Department of Infectious Diseases,Union Hospital Affiliated with Huazhong University of Science and Technology,Wuhan 430022,China) |
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| Abstract: | Objective To explore the effect of hepatitis B virus X protein on the expression of SOCS3 in human hepatoma(HepG2) cell line and its possible mechanisms.Method HepG2 cells were transfected with FL1-145 HBx plasmid coding for HBx protein,then a series of concentrations of SRC inhibitor(PP2) were added to the transfected cells.The mRNA and protein level of HBx and SOCS3 were assessed by RT-PCR and Western blot,and the expression of p-SRC in transfected cells was analyzed by immunocytochemistry.Result HBx protein was expressed in HepG2 cells which were transfected with FL1-145HBx.The levels of SOCS3 and p-SRC were up-regulated when HBx protein was expressed in HepG2 cells.However,after treated with different concentrations of PP2,the SOCS3 protein expression in transfected HepG2 cells gradually decreased,and this was accompanied by the reduction of p-SRC.Conclusion HBx protein may promote the expression of SOCS3 protein through activating p-SRC in the HepG2 cells. |
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| Keywords: | Hepatitis B virus HBx protein SOCS3 protein SRC protein |
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