靛玉红、蛇床子素抗外阴阴道念珠菌病混合菌生物膜作用的研究

目的研究中药有效成分靛玉红、蛇床子素抗外阴阴道念珠菌病混合菌生物膜的作用。方法体外建立白念珠菌（Candidaalbicans）铜绿假单胞菌（Pseudomonasaeruginosa，P．a）混合菌生物膜（Biofilm，BF），XTT减低法及形态学观察白念珠菌混合茵生物膜的形成过程；形态学观察、活菌计数法评价中药有效成分靛玉红（indirubin）、蛇床子素（Ostho）对白念珠菌混合菌生物膜的最小抑膜浓度（SMIC），并经扫描电镜确认。结果白念珠菌混合菌48h能形成成熟的生物膜；62．5mg／L浓度的靛玉红能抑制白念珠菌混合菌生物膜的形成。500mg／L浓度的蛇床子素未见有抑制白念珠菌混合菌生物膜的作用。结论靛玉红由于具有抗生物膜的作用，可用于预防外阴阴道念珠菌病的复发。

The effects of indirubin and Osthol against mixed biofilms of vulvovaginal candidiasis

Objective To investigate the effects of Chinese Herbal Drugs indirubin and Osthol against mixed biofilms of vulvovaginal candidiasis.Method C.albicans and P.aeruginosa were mixed,then the mixed biofilms were developed in vitro.XTT reduction assay and morphological observation were performed to learn the formation process of C.albicans mixed biofilms.Morphological observation and CFU counting were employed to evaluate the sessile minimal inhibitory concentrations(SMICs) of indirubin and Osthol on C.albicans mixed biofilms biomass and confirmed by SEM.Result Mature mixed biofilms of C.albicans and P.aeruginosa were developed after 48 h in vitro.SMICs of indirubin and Osthol against C.albicans mixed biofilms biomass were 62.50 mg/L and > 500 mg/L,respectively.Conclusion Indirubin displayed potential to inhibit mixed biofilms of vulvovaginal candidiasis,thus can be used to prevent the recurrence of vulvovaginal candidiasis.